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Scientific Backing

A brief Bibliography on why Third Life makes you awesome. Research only.

Brainy Bibliography

For all you supplement sleuths, here's the research (plus many, many trial-and-error experiments on ourselves) that led us to the ingredients you've come to know and love in Third Life Health. Below, you'll find a slew of clinical studies highlighting the benefits of:

Cognizin – boosts memory and attention

Agmatine Sulfate – supports mood and neuroprotection

Choline Bitartrate– improves cognition and reduces neuroinflammation

Caffeine + L-Theanine – sharpens focus, enhances alertness, and lifts mood

Dive in and see the science that powers Third Life Health.

Citicoline (Cognizin®)

Improves Focus, Memory, and Alertness while promoting Healthy Brain Aging

  • Study Summary:
    In a 12-week randomized, double-blind, placebo-controlled study, 100 healthy adults (aged 50–85) with age-associated memory impairment received either 500 mg Cognizin® citicoline or placebo.


    Findings:
    The citicoline group showed significantly greater improvements in both episodic memory (Paired Associate test) and composite memory scores than the placebo. The study suggested citicoline supplementation supports memory function in healthy aging.

    Read Study Here

    Nakazaki, et al. (2021). "Citicoline and Memory Function in Healthy Older Adults: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial" Journal of Nutrition.

  • Study Summary:
    Randomized, double-blind, placebo-controlled study, 60 healthy women aged 40–60, 250mg or 500mg citicoline for 28 days.

    Findings:
    Both doses improved attention, suggesting citicoline enhances cognitive inhibition and attentional performance.

    Read Study Here

    McGlade E, et al. (2012). "Improved Attentional Performance Following Citicoline Administration in Healthy Adult Women." Food and Nutrition Sciences

  • Summary:
    Randomized study, 28 days of citicoline (250 or 500 mg) vs placebo in 75 healthy adolescent males.

    Findings:
    Overall, citicoline supplementation improved attentional performance, psychomotor function, and inhibitory control in adolescent males.

    Read Study Here

    Agoston AM et al. (2019) "The Effect of Citicoline Supplementation on Motor Speed and Attention in Adolescent Males." Journal of Attention Disorders

Choline Bitartrate

Improves Attention, Cognition and Coordination. While Reducing Neuroinflamation, Cognitive Impairment, and the effects of Sleep Deprivation.

  • Study Summary:
    Cross-sectional analysis of 2,393 U.S. adults over 60, NHANES 2011–2014.

    Findings:
    Moderate choline consumption was linked to better cognitive outcomes in older adults.

    Read Study Here

    Liu L, et al. (2021) "Choline Intake Correlates with Cognitive Performance among Elder Adults in the United States." Behavioral Neurology.

  • Summary:
    Animal study in young mice fed choline bitartrate.

    Findings:
    Choline protected against sleep deprivation–induced cognitive deficits.

    Read Study Here

    Si-Yu Huang et al. (2025) "Choline alleviates cognitive impairment in sleep-deprived young mice." Redox Biology.

  • Summary:
    Placebo-controlled crossover, 28 healthy adults, 2g choline bitartrate.

    Findings:
    Choline enhances action precision.

    Read Study Here

    Naber, M. et al. (2015) "Improved human visuomotor performance after choline supplementation." Scientific Reports.

Caffeine + L-Theanine

Improves overall Cognition and Attentional Enagegment.

  • Summary:
    Randomized, placebo-controlled, four-way crossover in five boys (ages 8–15) with ADHD.

    Findings:
    The l-theanine–caffeine combination supported cognitive performance and attentional control.

    Read Study Here

    Kahathuduwa, C.N. et al. (2020) Nature: Scientific Reports.

  • Summary:
    Randomized, placebo-controlled, crossover, 27 healthy adults.

    Findings:
    The caffeine–L-theanine combination enhances performance beyond caffeine alone.

    Read Study Here

    Owen GN, et al. (2008) Nutritional Neuroscience.

  • Summary:
    Double-blind, placebo-controlled crossover, 24 adults.

    Findings:
    Caffeine improved attention and mood.

    Read Study Here

    Dodd FL, et al. (2015) Psychopharmacology.

Agmatine Sulfate

Modulates nitric oxide, increasing blood flow, enhancing mental clarity, stabilizing neural communication, and improving mood.

  • Summary:
    Preclinical study in mice.

    Findings:
    Agmatine as a potential candidate for rapid-onset antidepressant therapy.

    Read Study Here

    Valverde AP et al. (2021) World Journal of Psychiatry.

  • Summary:
    Mouse model of depression, forced swim test.

    Findings:
    Agmatine promotes faster improvements in emotional resilience and mental state.

    Read Study Here

    Meylan, E. et al. (2016) Nature: Translational Psychiatry.

  • Summary:
    Animal studies on neuropathic pain and nitric oxide signaling.

    Findings:
    Agmatine supports more stable neural communication.

    Read Study Here

    Rosenberg ML et al. (2018) Nutrients.